Figure 3 | Scientific Reports

Figure 3

From: Combining amplicon sequencing and metabolomics in cirrhotic patients highlights distinctive microbiota features involved in bacterial translocation, systemic inflammation and hepatic encephalopathy

Figure 3

Network and PICRUSt analysis of peripheral and portal blood microbiota in cirrhotic patients. Network analysis was performed on portal (panel A) and peripheral (panel B) blood, taking into account OTUs (within square brackets) with mean relative abundance ≥0.5%. Network properties as in Fig. 2. The crosscorrelation heatmap (panel C) depicts interrelationships and was built using Pearson coefficients, with a white star indicating a significant correlation (P < 0.05 after FDR correction). PICRUSt analysis (panel D) shows differences, among the four cirrhotic patient datasets, in the sequence numbers (expressed as % of the total) relative to three KOrths involved in bacterial iron transport: the peripheral and portal blood are significantly enriched in these three genes (Kruskal-Wallis test, Benjamini-Hochberg FDR q-value). Within boxes, stars represent the mean, while horizontal bars represent the median. Species percentage contributions to the three iron-related KOrths (panel E) were computed with PICRUSt and are reported as ‘normalized KO mean relative abundance (%)’ on the y-axis: normalization was performed according to the total number of sequences and number of samples for each cohort (biop_cirr, feces_cirr, periph_cirr, portal_cirr).

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