Figure 2
From: Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks
Recording of Ca2+ transients in isolated ventricular cardiomyocytes from mouse (A,B,E,F), rabbit (C,G), and pig (D,H). In VCM images (A–D), dark areas in the diagonal corners show the location of stimulating electrodes (the inter-electrode distance could vary from 170 to 300 μm). In A, the line along the axis of the cell is the region for line scan of Fluo-4 fluorescence (E, top). The fluorescence intensity plotted versus time (E, bottom) reveals Ca2+ transients elicited, in this example, by 5, 200-μs stimuli applied with 1-s intervals (red arrows). Panels F, G, and H show typical Ca2+ transients recorded from mouse, rabbit, and pig VCM, respectively. Pulse duration is indicated next to the traces.
