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Figure 2

From: An in vitro erythrocyte preference assay reveals that Plasmodium falciparum parasites prefer Type O over Type A erythrocytes

Figure 2

Selection of an optimal cell labelling combination for multiplex invasion of erythrocytes by P. falciparum. The impact of labelling erythrocytes with fluorescent dyes on infection by P. falciparum strain Dd2 was determined by flow cytometry. (A,B) Erythrocytes labelled with either CellTrace™ Far Red (DDAO), Calcein Red-Orange AM (CRO), Oregon Green 488 (OG) or Violet (CTV) were incubated individually (A) or altogether (B). (C,D) Erythrocytes labelled with 1 or 10 µM of DDAO or 10 µM of OG or a combination of both were incubated individually (C) or altogether (D). All cells were incubated in the presence of P. falciparum parasites for 48 h to allow rupture of schizonts and subsequent invasion of labelled and unlabelled erythrocytes. The cells were then stained with Hoechst 33342 and analyzed by flow cytometry to determine the parasitemia of each population and calculate the labelled invaded erythrocyte/unlabelled invaded erythrocyte ratio. Values greater than 1.0 indicate an increased susceptibility to invasion than unlabelled erythrocyte, while values smaller than 1.0 indicate an increased resistance to invasion.

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