Figure 3 | Scientific Reports

Figure 3

From: An in vitro erythrocyte preference assay reveals that Plasmodium falciparum parasites prefer Type O over Type A erythrocytes

Figure 3

Example gating scheme to determine parasitemia in labelled and unlabelled erythrocytes. Erythrocytes labelled with 1 or 10 µM of DDAO, 10 µM of OG or a combination of both were mixed and incubated with P. falciparum parasites for 48 h to allow rupture of schizonts and subsequent invasion of labelled and unlabelled erythrocytes. The cells were then stained with Hoechst 33342 and analyzed by flow cytometry to determine the parasitemia of each population. Individual erythrocyte populations were gated based on their MFI for both OG and DDAO, after excluding debris and doublet events.

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