Figure 3
In vivo imaging of thalamocortical axonal boutons with TPLSM, OEFM, SDCLM, and 8K-SDCLM. (a) Schematic illustration of imaging of TC axons in L1. (b) Representative two-photon image of TC axons that expressed GCaMP6s. The image is the average projection during the imaging. The image was cropped (85 × 85 µm) from the original 127 × 127 µm acquisition. Scale bar, 20 µm. (c,e) Representative OEFM (c) and SDCLM (e) imaging of tdTomato-expressing TC axons in the same cortical field. Scale bars, 200 µm. (d,f) Magnified images of white boxes in c (d) and e (f). Scale bars, 20 µm. (g) 8K-SDCLM imaging of a TC axon that expressed GCaMP6s. The image is the mean projection of frames in which the frame-averaged fluorescence exceeded its time-average value plus 2S.D. Scale bar, 200 µm. (h) Magnified images of blue and orange boxes in g. Scale bars, 20 µm.
