Figure 6
Highly correlated activity of axonal boutons on putative same axons scattered over the 1 mm2 cortical area. (a,b) Maps of correlation coefficients calculated by the pixel-wise correlation analyses. Magenta (a) and green (b) arrows show each seed. Scale bar, 100 µm. (c) Composite image of a and b, indicating no overlap. (d) Top, magnified image of a putative single axon, both ends of which are arrowed as “d” in c. White asterisks show peaks with highly correlated pixels detected manually. Scale bar, 50 µm. Bottom, correlation coefficients with seed 1 along the axonal arbor in the top panel. Blue asterisks correspond to white asterisks in the top panel. (e) Correlation coefficients with seed 1 (e1) and seed 2 (e2 and e3) were plotted along three putative axons. Both ends of each putative axon are arrowed as “e1”, “e2”, and “e3” shown in c. Blue asterisks show peaks with highly correlated pixels detected manually. “1” and “Seed 2” indicate pixels indicated by the arrowhead “1” and the arrowhead “Seed 2” in b, respectively. (f) Magnified images of the axonal boutons indicated by the seed and numbered arrowheads in a and b. Scale bar, 2 µm. The center pixels in the left-most images are seed 1 (top) and seed 2 (bottom). (g) Normalized ΔF/F traces of the axonal boutons shown in f. ΔF/F averaged over nine pixels at the center of each bouton was denoised using the CNMF algorithm. (h) Matrix of correlation coefficients for the activities between pairs of 12 boutons shown in g.
