Figure 2 | Scientific Reports

Figure 2

From: Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system

Figure 2

Culture of human hepatocytes as 3D organoids leads to long term survival, loss of mature hepatocyte markers, and enrichment in liver progenitors markers. (A) The expression of mature hepatocytes markers was analyzed by qPCR in adherent hepatocytes at 24, 48 and 72 h after plating (Adh), or in hepatocyte organoids (3D) after 2–3 passages (t1; around 40 days after plating) and 2 weeks later (t2). (B) The expression of markers of liver progenitors was analyzed in the same conditions, as well as Ki67 proliferation marker (C). The expression was normalized compared to the expression in adherent human hepatocytes 24 h post-plating. (N = 3).

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