Figure 5 | Scientific Reports

Figure 5

From: Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system

Figure 5

Induction of differentiation of 3D organoids into mature hepatocytes is more efficient if cultured in Matrigel suspension. The mRNA expression level of different liver progenitor/mature hepatocyte/proliferation markers was analyzed by qPCR, in 3D culture of human hepatocytes inside Matrigel drops (3D-D – black bars) or in Matrigel suspension (3D-S – white bars), in expansion (EM) or differentiation media (DM). (N = 3).

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