Figure 5 | Scientific Reports

Figure 5

From: Oligomeric states of microbial rhodopsins determined by high-speed atomic force microscopy and circular dichroic spectroscopy

Figure 5

Oligomeric states of XeR and microbial rhodopsins from archaea. (left panels) Typical HS-AFM images of (a) NpHR, (b) NpSRII, (c) PoXeR and (d) ASR reconstituted in the lipids. Frame rate, 1 fps. The average numbers of oligomers observed in the lipid particles were (NpHR) 9.1 ± 7.8 (mean ± s.d. for 201 trimers in 22 lipids), (NpSRII) 45.5 ± 31.3 (mean ± s.d. for 273 trimers in 6 lipids), (PoXeR) 50.0 ± 20.4 (mean ± s.d. for 150 trimers in 3 lipids), (ASR) 4.1 ± 1.7 (mean ± s.d. for 115 trimers in 28 lipids). Dotted triangles on the images indicate the trimer units. (middle panels) Magnified views of trimers for each microbial rhodopsin are shown with the number of subunits (1–3). (right panels) CD spectra of reconstituted (a) NpHR, (b) NpSRII, (c) PoXeR and (d) ASR in the lipids.

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