Figure 1

Illustration of the OCT scanning protocol and image analyses performed in the study. At each measurement session high resolution chorioretinal OCT images (each consisting of the average of 40 B-scans) were collected using a “cross” scanning protocol to capture 20° wide horizontal and vertical, foveal centred cross-sectional images (top panel). Each cross-sectional OCT image was analysed using semi-automated software to segment the retinal pigment epithelium (RPE, green line) and the chorioscleral interface (CSI, blue line) (bottom panel). These data were then used to calculate the subfoveal choroidal thickness; the foveal (F) region choroidal thickness (average thickness across the central 1 mm region); and the macular choroidal thickness (average thickness in a 2 mm region adjacent to the foveal region) in the superior (S), inferior (I), temporal (T) and nasal (N) regions.