Figure 3 | Scientific Reports

Figure 3

From: Loop-mediated Isothermal Amplification-Single Nucleotide Polymorphism Analysis for Detection and Differentiation of Wild-type and Vaccine Strains of Mink Enteritis Virus

Figure 3

Detection limit of LAMP-SNP assay. (A) Electrophoresis. Lane M: DNA Marker DL2000 (TaKaRa, Dalian, China). Lanes 1–8 represent 2 × 106–2 × 10−1 copies/mL of pT-MEV-NS1 plasmid, respectively. (B) Visual inspection. Tubes 1–8 represent 2 × 106–2 × 10−1 copies/mL of pT-MEV-NS1 plasmid, respectively. Both methods exhibited the same results, the detection limit was 101 copies/mL, with no differences between electrophoresis and visual inspection. Full-length gels and tubes are presented in Supplementary Fig. 3A,B.

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