Table 2 Primers were used in this study for PCR, MEV-VP2-LAMP, and LAMP-SNP (MEV-NS1-LAMP).
Assays | Primers | Primer Sequence (5′-3′) | Source |
|---|---|---|---|
pT-MEV-NS1 plasmid construction | NS1 F | CGCGGATCCATGTCTGGCAACCAGTATA | This study |
NS1 R | CGGCTGCAGTTAATCCAAGTCGTCTCGAA | ||
MEV-VP2-LAMP | VP2 F3 | GTAAACCATGTAGACTAACACA | ref.15 |
VP2 B3 | AACCTCAGCTGGTCTCAT | ||
VP2 FIP (F1c + F2) | TCCTTCAGATTGAGGCAAAGAATTT + TACATGGCAAACAAATAGAGC | ||
VP2 BIP (B1c + B2) | AGGAGTTCAACAAGATAAAAGACGT AGTAGCTTCAGTAATATAGTCTGT | ||
LAMP-SNP (MEV-NS1-LAMP) | NS1 F3 | TAGAGACACAAGCGGCAA | This study |
NS1 B3 | CCTCTATTTCGGACCACG | ||
NS1 FIP (F1c + F2) | CTGGAGTACTCCACGGTTCC TCCTCAGAGTCAAGACCA | ||
NS1 BIP (B1c + B2) | GATACGCCTATTGCAGAAACT TTGCACGTCTTTGTGAGT |
