Table 2 Preliminary evaluation of sensitivity, specificity, positive and negative predicted values for the Nb44/Nb42 LFA performed on plasma samples from experimentally infected cattle.

From: Development of a Nanobody-based lateral flow assay to detect active Trypanosoma congolense infections

 

Cattle sera infected with

T. congolense

Naive

T. vivax

Positive

19

1

2

Negative

5

14

20

Total

24

15

22

Sensitivity ± 95% CI (%)

79.17 (19/24) ± 16.25

Specificity ± 95% CI (%)

91.89 (34/37) ± 8.80

PPV ± 95% CI (%)

86.36 (19/22) ± 14.34

NPV ± 95% CI (%)

87.18 (34/39) ± 10.47

  1. The samples were obtained from a stored GALVmed plasma sample collection at Clinvet, South Africa. The collection contains plasma samples of Holstein cows that were experimentally infected with T. vivax STIB 719 or T. congolense KONT 2/133. Because these animals were involved in drug development programs, they were treated as soon mild disease symptoms developed combined with an observed hematocrit value lower than 25%.
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