Figure 2
From: Regulation of CD4+CD8−CD25+ and CD4+CD8+CD25+ T cells by gut microbiota in chicken
Expression of IL-10 and IFN-γ mRNA among CD4+ T cell subsets in cecal tonsils of ABX-treated chickens. Chickens were given water containing antibiotics at hatching for 7 days, and cecal tonsils were taken. Single cells from cecal tonsils were stained with anti-chicken CD4, CD8α, and CD25 antibodies. Each subset of CD4+ T cells was sorted using an ARIA II FACS sorter. The mRNA was extracted from each subset, and the expression levels of (A) IL-10 and (B) IFN-γ were determined by RT-qPCR. Data were obtained from three chickens in each group and presented as the mean ± SD. Asterisks indicate significant differences between Con and ABX. *P < 0.05 and **P < 0.01.
