Figure 3
From: Regulation of CD4+CD8−CD25+ and CD4+CD8+CD25+ T cells by gut microbiota in chicken

Changes in microbial number (colony forming units; CFU), CD4+CD8−CD25+, and CD4+CD8+CD25+ T cells in cecal tonsils of ABX-treated chickens after co-housing with control chickens. Chickens were treated with ABX at hatching for 7 days and then co-housed with control chickens for 7 days. (A) CFU was measured from cecal contents (1 mg/ml) at 1/4 (6 hours), 1, 3, and 5 days after co-housing. (B) Proportions and numbers of CD4+CD8−CD25+ and CD4+CD8+CD25+ T cells in cecal tonsils were analyzed by flow cytometry after co-housing. Data were obtained from more than six chickens in each group and presented as the mean ± SD. Significant differences between Con and ABX are shown by asterisks, and data are representative of three independent experiments. *P < 0.05 and ***P < 0.001.