Figure 2 | Scientific Reports

Figure 2

From: MCC950, a specific small molecule inhibitor of NLRP3 inflammasome attenuates colonic inflammation in spontaneous colitis mice

Figure 2

MCC950 inhibits NLRP3 inflammasome activation in colonic explants. (a) Production of IL-1β from Winnie proximal and distal colons stimulated with 10 ng/ml LPS and treated with MCC950 (MCC) (0.001–10 µM) and Glyburide (Gly) 200 µM as measured by ELISA. (b)Percentage of IL-1β release of Winnie proximal and distal colons stimulated with LPS no treatment compared to treated with MCC950 (10 µM) as measured by ELISA. (c) Production of TNF-α for proximal and distal colonic explant supernatants treated with MCC950 (1 µM) and glyburide 200 µM as measured by ELISA. Data are expressed as the mean ± SEM of five independent experiments carried out in duplicates. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA with Tukey’s post-hoc test). (d) Western blots of tissue lysates and supernatants from proximal and distal colons stimulated with 10 ng/ml LPS and treated with MCC950 (1 µM) or glyburide (200 µM). These results are representative of three independent experiments.

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