Figure 1 | Scientific Reports

Figure 1

From: Prestressed cells are prone to cytoskeleton failures under localized shear strain: an experimental demonstration on muscle precursor cells

Figure 1

Principle of living cell indentation and FIC collection with an AFM cantilever tip. (a) Fluorescence images of myoblast cells (Top) and myotubes (Bottom) showing their nuclei (DAPI - blue), MFs (phalloidin alexa fluor 488 - green) and MTs (β-tubulin Cy3-conjugate - red) (see the Supplementary Information for further description of the staining). (b) Sketch of the AFM set-up. (c) Typical load (red) and unload (green) FICs collected on a myoblast with a 1 μm/s cantilever scan velocity: (1) work integrals during load W l and unload W u ; (2) the square root of the FIC versus the distance to contact Z − Z c highlights two linear regimes G g and G i bounding the loading FIC; (3) parabolic curves corresponding to G g (resp. G i ) and their work integrals W g (resp. W i ) (see the Section Methods).

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