Figure 1
From: Development of in vitro and in vivo neutralization assays based on the pseudotyped H7N9 virus
Development and optimization of PBNA. Panels (A,B) Selection of cell lines. The X-axis indicates the time (hours) after H7N9 pseudovirus infections while the Y-axis represents the RLU in the luciferase assay. Panel (A) 10000 TCID50 virus/30,000 cells; Panel (B) 100TCID50/30,000 cells. Panels (C,D) Determination of assay time points. The X-axis indicates serial dilutions of H7N9 inocula. Panel (C) 10000 cells/well. Panel (B) 30000 cells/well. Panels (E) Determination of MDCK cell density. MDCK cells were seeded from 5000 to 100000 cells in 96-wells plate and luciferase activity was detected at 48 h. Panel (F) Determination of H7N9 pseudovirus concentrations. Different virus dose were compared in neutralizing assay. Each data represents the mean luciferase activities of 3 replicates.
