Figure 4
From: Development of in vitro and in vivo neutralization assays based on the pseudotyped H7N9 virus
Development and optimization of bioluminescent imaging model. Panel (A) Imaging analysis of five- to six-week-old Balb/c mice inoculated with H7N9 pseudotype virus (5.62 × 106 TCID50 per mouse) via intranasal (IN.), intravenous (IV.), intrapleural (IPL.), or intraperitoneal (IP.) routes (n = 4/group). Panel (B) Selection of mouse strains: different mouse strains infected intraperitoneally with 5.62 × 106 TCID50 pseudovirus were imaged on day 4 (n = 4/group). Panel (C) Balb/c mice was imaged with intraperitoneally infected H7N9 pseudovirus (5.62 × 106 TCID50) on day 1, 2, 3, 4, 6 or 8 (n = 4/group). Panel (D) Results of photon flux obtained with different mouse strains on day 4. All results are shown as mean ± s.e.m (n = 4/group). Panel (E) Values of photon flux from day 1 to 8 for Balb/c mice, with results shown as mean ± s.e.m (n = 4/group).
