Figure 4 | Scientific Reports

Figure 4

From: Simultaneous purification of dihydrotanshinone, tanshinone I, cryptotanshinone, and tanshinone IIA from Salvia miltiorrhiza and their anti-inflammatory activities investigation

Figure 4

The inhibitory nitrite level and nitric oxide (NO), TNF-α, and IL-6 release of TTS in LPS-stimulated RAW264.7 cells. (A) Cells were pretreated with DTAN, TANI, CTAN, TANA, and TTS for 1 h before LPS (1 μg/mL) co-culture for 24 h. The nitrite levels of those samples were determined by Griess assay. (B) Cells were individually treated with DTAN, TANI, CTAN, TANA, and TTS for 6 h and then labeled with DAF-FM (1 μM) for another 1 h. The NO release was determined by the flow cytometry. (C) The quantitative fluorescence intensity was statistically determined. (D) Cells were treated with DTAN, TANI, CTAN, TANA, and TTS respectively for 24 h. The cell viability was investigated by MTT assay. (E and F). Cells were pretreated with TTS for 1 h and then stimulated with/without LPS (1 μg/mL) for 16 h. The ELISA assay was employed to detect levels of TNF-α and IL-6 in the culture medium. *p < 0.05, **p < 0.01, and ***p < 0.01 versus LPS-treated group.

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