Figure 5

TTS suppressed xylene-induced ear oedema and rescued LPS-induced septic shock in mice. (A) Ear oedema was established by xylene injection. Pretreatment with TTS (80 mg/kg) for 2 h before xylene injection. One hour later, ear weight was measured following the Methods. Values are shown mean ± SD (n = 10). (B) Histopathological examination of the ear sections. a. The left ear of mice not treated with ISO was regarded as a vehicle control. b. The right ear of mice treated with xylene (30 μL) but not with TTS was regarded as the model control. c. The left ear of a mouse pretreated with TTS (80 mg/kg, i.p.) for 2 h but not treated with xylene. d. The right ear of a mouse pretreated with TTS (80 mg/kg, i.p.) for 2 h and then treated with xylene (30 μL) for another 1 h. The ear tissue samples were stained with H&E as described in the Methods section. (H&E, original magnification, 400×). (C) 20 mice per group pretreated with vehicle or TTS (80 mg/kg, i.p.) for 2 h before LPS (20 mg/kg, i.p.) injection. DEX (5 mg/kg, i.v.) was used as positive control. Survival rates of these mice were observed for next 132 h. ^*P < 0.05 compared to xylene alone group.