Figure 9

Plant protection induced by SRBs on wild type and hormonal Arabidopsis mutants. Plants were spray-elicited with 350 µM SRBs or 0.5% ethanol (control) 48 hours prior pathogen inoculation. SRB protection in wt Arabidopsis (A), jar1 and sid2 mutants (C) against Pst. Arabidopsis clumps (15 plants per pot) were spray-inoculated and bacterial titers of infected tissues were determined three days after inoculation. Results represent the mean and SEM of three independent experiments with n ≥ 24 (A) and n ≥ 20 (C). The differences were analyzed by the non-parametric Kruskal-Wallis test (p < 0.05) for (A) and by the Mann Whitney test for (C). SRB protection in wt Arabidopsis against Botrytis cinerea (B). Arabidopsis leaf discs from six-weeks-old plants were drop-inoculated with 10 µL of B. cinerea conidia suspension (10⁵ conidia/mL) and incubated in Petri dishes containing water-wet filter paper. The area of necrosis was determined with ImageJ software four days after inoculation. Data represent the mean and SEM of three independent experiments with n ≥ 20 for each experiment. No significant differences were observed by the non-parametric Kruskal-Wallis test (p < 0.05).