Figure 1 | Scientific Reports

Figure 1

From: Impairment of photoreceptor ribbon synapses in a novel Pomt1 conditional knockout mouse model of dystroglycanopathy

Figure 1

Generation of Pomt1 retinal cKO mice. (a) Strategy for the generation of Pomt1 null alleles. The Pomt1 targeted allele was converted into a Pomt1 floxed allele by flippase-mediated recombination removing the DNA segment flanked between FRT sites. In the resulting Pomt1 floxed allele, exons 3 and 4 were flanked by loxP sites and were removed by Cre recombination to generate the Pomt1 null allele (Pomt1−). Arrowheads indicate the position of primers to discriminate Pomt1 wild-type and floxed alleles (used in b). Arrows show the annealing position of primers designed to assess Cre-mediated recombination in mouse retinas (used in Fig. 2a). The Crx-Cre gene fusion and the primers (arrows) used for its genotyping are represented at the bottom of the panel. (b) Results of PCR genotyping of Pomt1 alleles (upper panel) and of the Crx-Cre gene fusion (lower panel). This analysis allowed the discrimination between Pomt1 homozygous (wild-type or floxed) and heterozygous genotypes. The Crx-Cre genotyping yields a 797 bp band for the gene fusion and a 324 bp band for the control IL-2 gene. Genotypes defined by the results are indicated above each lane. First lane, DNA ladder. Uncropped gel image is presented in Supplementary Fig. S6a.

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