Figure 3
Functional retinal responses in Pomt1 cKO mice. ERG responses recorded from representative control (black lines) and Pomt1 cKO mice (gray lines) are shown (a–c). (a) Scotopic ERG rod response elicited by a −1.5 log cd∙s∙m−2 flash stimulus in control (Pomt1+/lox) and Pomt1 cKO mice. (b) Mixed ERG rod plus cone responses showing the negative (a-wave) and positive (b-wave) deflections elicited by a 1 log cd∙s∙m−2 flash stimulus under scotopic conditions in control (Pomt1+/lox) and Pomt1 cKO mice. (c) Photopic ERG cone responses elicited by a 1.6 log cd∙s∙m−2 flash stimulus in control (Pomt1+/lox) and Pomt1 cKO mice. (d,e) Intensity-response curve analyses. The b-wave response amplitude (mean ± SEM, n = 18) is represented as a function of light intensity (log cd∙s∙m−2) from recordings obtained under scotopic (d) or photopic (e) conditions. Individuals analyzed included 6 Pomt1lox/lox, 6 Pomt1+/− and 6 Pomt1+/lox mice (control group), in comparison to 18 Pomt1 cKO mice (mutant group). Response amplitudes showed statistical differences (***p < 0.001) (two-way ANOVA) between control and Pomt1 cKO mice. (f) Implicit times of the b-wave averaged in rod, mixed and cone responses (mean ± SEM, n = 18; genotypes as in d,e) also showed statistical differences (***p < 0.001) between the two animal groups. Separate results obtained for each of the three control genotypes are presented in Supplementary Fig. S3.
