Figure 1
Chloroquine (CQ) or amodiaquine (AQ) restored pAMPKα and pPGC1α expression in hRPTCs under high-glucose (HG) conditions. 3 × 105 cells were seeded in a 60 mm dish with 5–30 mM D-glucose and cultured for 6 hours. To explore the effects of the drugs on hRPTCs subjected to HG conditions, CQ (200 μM) or AQ (10 μM) were treated for 1 hour before changing culture medium into 30 mM D-glucose. Cell extracts were immunoblotted with anti-phospho-AMPKα, anti-AMPKα, anti-phospho-PGC1α, anti-PGC1α and anti-βactin antibody. (A) Western blot analyses revealed that AMPKα phosphorylation was increased in hRPTCs treated with CQ or AQ under HG conditions. Band intensities representing phospho-AMPKα and AMPKα expression levels were converted into densitometry using ImageJ software in the ratio of phospho-AMPKα to AMPKα. Results are means ± SEM. for experiments in triplicate. (B) Representative immunoblot data showed that PGC1α phosphorylation was also enhanced in hRPTCs after treatment with CQ or AQ under HG conditions. Band intensities representing phospho-PGC1α and PGC1α expression levels were converted into densitometry using ImageJ software in the ratio of phospho-PGC1α to PGC1α. Results are means ± SEM. for experiments in triplicate. (*p < 0.05 vs 5 mM, #p < 0.05, ##p < 0.01 vs 30 mM).
