Figure 4

Effect of siAMPK on CQ- or AQ- induced mitochondrial biogenesis and homeostasis. (A) Expression levels of Tom20, Mfn1, and Drp1 in AMPK-knockdown hRPTCs under high glucose condition with or without treatment of CQ. Band intensities representing Tom20 and GAPDH expression levels were converted into densitometry using ImageJ software in the ratio of Tom20 to GAPDH. Band intensities representing Mfn1 and Drp1 expression levels were converted into densitometry using ImageJ software in the ratio of Mfn1 to Drp1. Results are means ± SEM. for experiments in triplicate. (**p < 0.01 vs 5 mM, ^#p < 0.05, ^##p < 0.01 vs 30 mM, ^†p < 0.05 vs 30 mM + siAMPK, ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 vs 30 mM + siAMPK + CQ). (B) Expression levels of Tom20, Mfn1, and Drp1 in AMPK-knockdown hRPTCs under high glucose condition with or without treatment of AQ. Band intensities representing Tom20 and GAPDH expression levels were converted into densitometry using ImageJ software in the ratio of Tom20 to GAPDH. Band intensities representing Mfn1 and Drp1 expression levels were converted into densitometry using ImageJ software in the ratio of Mfn1 to Drp1. Results are means ± SEM. for experiments in triplicate. (**p < 0.01 vs 5 mM, ^#p < 0.05, ^##p < 0.01 vs 30 mM, ^$p < 0.05, ^$$p < 0.01 vs 30 mM + siAMPK + AQ).