Figure 3

Pathogenicity of Dictyocoela spp.: (A) Gammarus varsoviensis infected with Dictyocoela muelleri. Pale-colored musculature indicates microsporidian infection (arrow). (B) Infected abdominal muscles (arrow) of the same specimens (Nomarski contrast). (C) Ultrathin section of muscle cell of Pontogammarus robustoides infected with D. berillonum. Sporophorous vesicles are located in the sarcoplasm neighboring myofibrils (mn). Deformation of host nucleus (hn) is observed. Single sporophorous vesicle (sv) is located outside the muscle cell in haemolymph. (D) Diplokaryotic sporont (sp) and sporophorous vesicles (sv) of D. muelleri, located in hemolymph of Dikerogammarus haemobaphes. Sporophorous vesicles (sv) adhering to haemocyte (hc) are visible. (E). Sporophorous vesicle of Dictyocoela berillonum (arrow) inside the cytoplasm of uninucleate haemocyte of P. robustoides. Single nucleus of haemocyte (hn) contains two nucleoli. Scale bars: 2.0 mm (A), 0.2 mm (B); 3.5 µm (C); 2.0 µm (D); 4.5 µm (E).