Table 2 Antibody probing conditions used for western blot analyses.
Target | Abbreviation | Company | Host | Blocking Conditions | Primary Antibody Conditions |
|---|---|---|---|---|---|
Beta III Tubulin | β-tubulin III | Abcam (Cambridge, MA) | Rabbit | 30 min in 5% milk in 0.05% T-TBS | 1:30,000 in 5% milk in 0.05% T-TBS |
Excitatory Amino Acid Carrier 1 | EAAC1 (EAAT3) | AbBiotec (San Diego, CA) | Rabbit | 1 hr in 5% milk in 0.05% T-TBS | 1:20,000 in 5% milk in 0.05% T-TBS |
GABA Transporter 3 | GAT3 | Novus Biologicals (Littleton, CO) | Rabbit | 1 hr in 5% milk in 0.1% T-TBS | 1:4000 in 5% milk in 0.1% T-TBS |
Glial Fibrillary Acidic Protein | GFAP | Dako (Carpinteria, CA) | Rabbit | 30 min in 5% milk in 0.05% T-TBS | 1:30,000 in 5% milk in 0.05% T-TBS |
Glutamate-Aspartate Transporter | GLAST (EAAT1) | Cell Signaling (Danvers, MA) | Rabbit | 1 hr in 5% milk in 0.1% T-TBS | 1:10,000 in 5% BSA in 0.1% T-TBS |
Glutamate Receptor 1 | GluA1 (GluR1) | Millipore (Billerica, MA) | Rabbit | 30 min in 5% milk in 0.05% T-TBS | 1:1000 in 5% milk in 0.05% T-TBS |
Glutamate Receptor 2 | GluA2 (GluR2) | Millipore (Billerica, MA) | Mouse | 1 hr in 5% BSA in 0.1% T-TBS | 1:1000 in 5% BSA in 0.1% T-TBS |
Glutamate Transporter-1 | GLT1 (EAAT2) | Novus Biologicals (Littleton, CO) | Rabbit | 1 hr in 5% milk in 0.1% T-TBS | 1:10,000 in 5% BSA in 0.1% T-TBS |
Glyceraldehyde 3-phosphate dehydrogenase | GAPDH | Calbiochem (Billerica, MA) | Mouse | 30 min in 5% milk in 0.05% T-TBS | 1:30,000 in 5% milk in 0.05% T-TBS |
Metabotropic Glutamate Receptor 1 | mGluR1 | BD Bioscience (Franklin Lakes, NJ | Mouse | 30 min in 5% milk in 0.05% T-TBS | 1:2500 in 5% milk in 0.05% T-TBS |
N-methyl D-aspartate receptor subtype 2A | GluN2A (NR2A) | Millipore (Billerica, MA) | Rabbit | 1 hr in 5% milk in 0.1% T-TBS | 1:10,000 in 5% milk in 0.1% T-TBS |
N-methyl D-aspartate receptor subtype 2B | GluN2B (NR2B) | Calbiochem (Billerica, MA) | Rabbit | 30 min in 5% milk in 0.05% T-TBS | 1:1000 in 5% milk in 0.05% T-TBS |
