Figure 2

Deconvoluted ESI-MS spectra of HRGP330 incubated at varying Zn2+:peptide ratios. Peptide samples (15 μM) in 10 mM ammonium acetate (pH 7.40) were titrated with zinc acetate and were analysed on a maXis QToF mass spectrometer (Bruker Daltonics). The isotopic distribution for the 1:1 Zn-HRGP330 complex is shown on the right hand side together with a comparison to the theoretical model.