Figure 3 | Scientific Reports

Figure 3

From: Native electrospray mass spectrometry approaches to probe the interaction between zinc and an anti-angiogenic peptide from histidine-rich glycoprotein

Figure 3

Stacked plot of 700 MHz 1D 1H-NMR spectra showing the fingerprint region of HRGP330 in the presence of varying amounts of ZnCl2. 0.5 mM samples were prepared in 50 mM Tris[D11], 50 mM NaCl with 10% D2O at pH* 6.2 and 278 K. Aromatic protons from His residues are annotated, but significant overlapping of peaks meant they could not be assigned unambiguously in 2D spectra (Figs S3 and S5). A peak corresponding to formate (chemical shift standard) is denoted by “f”.

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