Figure 4

Effect of Zn2+ binding on the ATD of the 5+ charge state of HRGP330. ATD peaks of Zn2+ bound species, given in ms. The sample consisted of 10 µM HRGP330 in 10 mM ammonium acetate (pH 7.4) and 50 μM Zn. The Zn1-HRGP330 peak identified in the sample was too low in intensity to give reliable data. The trace for the apo-peptide was acquired on a zinc-free sample. ATD data for all charge states for apo-HRGP330 are shown in Fig. S6.