Table 3 Mean number of SNP loci accurately called and miscalled for the different combination of tissue pools.

From: High sample throughput genotyping for estimating C-lineage introgression in the dark honeybee: an accurate and cost-effective SNP-based tool

Tissue pools (# of replicates)

Accurate SNPs

Miscalled SNPs

i

ii

iii

iv

v

1 Mel + 1 Hyb (3)

97.0

1.0

4.7

4.7

5.3

0.3

2 Mel + 1 Hyb (2)

81.0

0.0

3.5

5.0

21.0

0.5

3 Mel + 1 Hyb (2)

83.5

0.0

5.5

5.0

17.5

0.0

7 Mel + 1 Hyb (2)

58.0

0.0

4.5

5.0

47.0

0.5

1 Mel + 1 Car (3)

85.0

0.3

4.3

4.0

5.3

11.0

1 Car + 1 Hyb (3)

101.7

0.3

3.7

2.0

4.0

1.3

2 Car + 1 Hyb (2)

93.5

0.0

3.0

2.5

13.0

0.0

3 Car + 1 Hyb (2)

93.0

0.0

3.0

5.0

12.0

0.0

1 Buc + 1 Hyb (3)

102.7

0.0

3.0

1.3

4.7

0.7

  1. The sources of miscalling were (i) different alleles, (ii) higher DNA concentration, (iii) higher DNA concentration and the most frequent allele, (iv) the most frequent allele, and (vi) the least frequent allele. Mel - A. m. mellifera; Hyb – F1 hybrid; Car – A. m. carnica; Buc – Buckfast.
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