Figure 1 | Scientific Reports

Figure 1

From: Cross-talk among HMGA1 and FoxO1 in control of nuclear insulin signaling

Figure 1

Physical association between HMGA1 and FoxO1. (a) SDS-PAGE of 35S-FoxO1a bound to GST-HMGA1 resin. Lane 1, input; Lane 2, GST alone; Lane 3, labeled protein retained by GST-HMGA1 resin. (b) Immunoprecipitation (IP) of FoxO1 and HMGA1 by using the anti-HMGA1 antibody followed by immunoblotting with the anti-FoxO1 antibody (lanes 1-2), or the anti-HMGA1 antibody (lanes 3-4), after reprobing the same transfer. Lanes 1 and 3, 10 ng of pure HMGA1; lanes 2 and 4, HepG2 nuclear extract (NE; 500 µg). Lanes 1 and 3, protein was directly applied to the gel without binding to and elution from protein A beads. Cropped blots are shown in the figures. Full-length blots are presented in Supplementary Fig. S1.

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