Figure 2
From: Cross-talk among HMGA1 and FoxO1 in control of nuclear insulin signaling
HMGA1 and FoxO1 fusion protein expression and IGFBP1-Luc reporter gene assay following transfection of effector vectors for YFP-HMGA1 and CFP-FoxO1 into HEK-293 cells. (a) Representative WBs of YFP-HMGA1 and CFP-FoxO1 fusion proteins are shown in the left and right upper panels of the figure, respectively. Functional activity of both fusion proteins is shown in reporter gene assays, using HEK-293 cells cotransfected with the IGFBP1-Luc reporter construct in the presence of either HMGA1/YFP-HMGA1 (left) or FoxO1/CFP-FoxO1 (right) effector vectors (0.1 µg each). Cropped blots are shown in the figure. Full-length WBs are presented in Supplementary Fig. S1. (b) FRET. Distribution of YFP-HMGA1, CFP-FoxO1, and FRET signal in starved HEK-293 cells. Distribution of maximum FRET signal is presented using a black-white lookup table.
