Figure 3
From: Cross-talk among HMGA1 and FoxO1 in control of nuclear insulin signaling
Functional significance of HMGA1/FoxO1 interaction. (a) Representative ChIP of the IGFBP1 promoter gene with anti-FoxO1 antibody (Ab) and qRT-PCR of ChIP-ed samples in HepG2 cells untreated or pretreated with distamycin A (100–150 µM), in the absence or in the presence of 10 nM insulin. *P < 0.05 vs untreated cells (slashed bars). Cropped gel is shown in the figure. Full-length ChIP is presented in Supplementary Fig. S1. (b) IGFBP1 suppression and FoxO1 phosphorylation in HepG2 cells under the same conditions as in (a). Conditioned medium samples and cell nuclear extracts were collected after 12 h insulin treatment and assayed by western blot (WB) for IGFBP1, HMGA1, total FoxO1, and phosphorylated pFoxO1. Densitometer scanning of IGFBP1 is shown in bar graphs. Results are expressed as percentages of the IGFBP1 protein production as obtained in the presence of vehicle alone. Cropped blots are shown in the figure. Full-length WBs are presented in Supplementary Fig. S1. *P < 0.05 vs vehicle alone.
