Figure 7
From: Cross-talk among HMGA1 and FoxO1 in control of nuclear insulin signaling
FoxO1 and IGFBP1 expression in liver from normal and Hmga1-deficient mice. Liver FoxO1 and Igfbp1 mRNA and protein levels were assayed in Hmga1 wild-type (Hmga1+/+) and knockout (Hmga1−/−) 12 h-fasted mice (n = 6), and mice refed for 4 h (n = 6) with a high carbohydrate meal after a 12-h fast. mRNA was measured by qRT-PCR and normalized to RPS9 mRNA abundance. *P < 0.05 vs fasted mice. Representative WBs of Hmga1, FoxO1, and Igfbp1 in both genotypes are shown in the autoradiograms. β-Actin, control of protein loading. Cropped blots are shown in the figure. Full-length WBs are presented in Supplementary Fig. S1.
