Table 1 The sequence of forward and reverse primers size (20–25 bp) used in the gene expression analysis.
Sr. No. | Enzyme | Primer sequence | |
|---|---|---|---|
Forward 5′→3′ | Reverse 3′→5′ | ||
1 | SOD | 5′GTCCACGCAGACCCTGATGT3' | 3′GAAGACCAATAATACCGCAAGCA5' |
2 | APOX | 5′CCGGTGAGAAGGAAGGTCTTC3′ | 3′CTTCCTCGTCAGCAGCGTATT5′ |
3 | CAT | 5′CTGACCCCCGCATCACA3′ | 3′ACGTTCAGACGGCTTGCAA5′ |
4 | DHAR | 5′CCAAAGGTGATGGGCTAAAGAG3′ | 3′ACATTGTACTAAAAGAAAGCAAGAGAAAAG5′ |
5 | MDHAR | 5′CCCAAAGCTAGCAAGAAGTCAAC3′ | 3′CTGTAGAGCGGCTTGAGCAA5′ |
6 | GR | 5′CACAGCAGCTGAGGAGTTTGTC3′ | 3′GACAGCTGTTTTAGCCTCAAGACTT5′ |
7 | Actin | 5′GGATCTCGAAGGGAGAGTACGA3′ | 3′TACCACACTCACCACCACGAA5′ |
