Table 3 Effect of 28-HBL concentrations (0, 10−6, 10−9, 10−12 M) on MDHAR, DHAR and GR activities of B. juncea L.

From: 28-homobrassinolide regulates antioxidant enzyme activities and gene expression in response to salt- and temperature-induced oxidative stress in Brassica juncea

Treatments

GR activity (U min−1 mg−1 proteins)

MDHAR activity (U min−1 mg−1 proteins)

DHAR activity (U min−1 mg−1 proteins)

Control

4 °C

44 °C

180 mM NaCl

10−6 M 28-HBL

10−9 M 28-HBL

10−12 M 28-HBL

10−6 M + 180 mM + 4 °C

10−6 M + 180 mM + 44 °C

10−9 M + 180 mM + 4 °C

10−9 M + 180 mM + 44 °C

10−12 M + 180 mM + 4 °C

10−12 M + 180 mM + 44 °C

24.23 ± 0.14l

45.21 ± 0.46h

48.98 ± 0.55g

46.20 ± 0.49h

29.56 ± 0.21j

36.91 ± 0.24i

28.40 ± 0.18k

64.97 ± 0.75d

68.66 ± 0.84c

72.31 ± 0.95b

85.43 ± 1.11a

53.00 ± 0.61f

62.32 ± 0.71de

23.04 ± 0.32e

29.12 ± 0.60c

33.31 ± 0.71b

30.35 ± 0.65c

26.58 ± 0.54d

27.17 ± 0.56cd

24.09 ± 0.36e

33.49 ± 0.72b

34.36 ± 0.75b

36.02 ± 0.80ab

38.50 ± 0.88a

28.74 ± 0.59cd

30.60 ± 0.67bc

9.34          ± 0.10j

14.37 ± 0.23f

17.31 ± 0.29d

15.71 ± 0.25e

12.08 ± 0.15h

13.59 ± 0.18fg

11.36 ± 0.14i

19.13 ± 0.34c

20.61 ± 0.36b

21.90 ± 1.39ab

24.10 ± 0.45a

18.31 ± 0.30cd

18.76 ± 0.32c

F-ratio12×26

417.4

60.58

49.20

  1. Under combinations of extreme temperature (4 °C, 44 °C) and NaCl salt (180 mM) stress in laboratory conditions.
  2. The data represented above are means ± S.E (n = 5). Different superscripted alphabetical letters within a column indicate significant differences from each other in all combinations (Tukey’s test, p ≤ 0.05).