Figure 8

IFT57-GFP fusion protein accumulates in the cortex and cytoplasm when cells are depleted of IFT46. (A1–A3) FT57-GFP fusion protein localised at basal bodies in cells treated with ND7 RNAi as a control before the first division. (B1–B3) IFT57-GFP fusion protein accumulated abnormally between basal bodies and in the cytoplasm of IFT46 RNAi cells before the first division, as shown with arrows. (C1–C3) IFT57-GFP fusion protein localised at basal bodies in cells treated with ND7 RNAi as a control for 24 h. (D1–D3) Accumulation of IFT57-GFP focused on the cytoplasm of IFT46 RNAi cells for 24 h, as shown with arrows. Bar: 10 µm.