Figure 4
From: The guanine nucleotide exchange factor Arhgef7/βPix promotes axon formation upstream of TC10
Active Cdc42 is not able to rescue the loss of Arhgef7. (a) Dissociated hippocampal neurons were transfected 6 h after plating with expression vectors for an shRNA against Arhgef7 (Arhgef7 RNAi) and GFP-Cdc42F28L or GFP (green). Neurons were analyzed at 3 d.i.v. by staining with an anti-MAP2 (red, dendrites) and the Tau-1 (blue, axons) antibody. The scale bar is 20 μm. (b) The formation of axons was analyzed at 3 d.i.v. by counting the number neurons without an axon (0, black) with a single axon (1, gray), and with multiple axons (>1, white) (ANOVA, Student’s t-Test, *p < 0.05, compared to controls, n.s is non significant for the indicated comparison, n = 3 experiments with at least 50 neurons counted per condition).
