Figure 2

Mesenchymal stem cell morphology on substrates. (a) SEM of hMSCs on flat Pt-BMG, 200 nm Pt-BMG, titanium, and tissue culture plastic, scale bar = 100 μm. (b) Representative images of hMSCs stained with phalloidin (cytoskeleton, red) and DAPI (nucleus, blue), scale bar = 50 μm. (c) FIB-SEM images of a hMSCs on 200 nm Pt-BMG, scale bar = 5 μm. (d–g) Quantification of cell area, perimeter, circularity, and elongation index (one-way ANOVA with post hoc Tukey HSD test, n = 50 cells, *p < 0.05). Error bars represent standard error of the mean (SEM).