Figure 3

Differentiation on substrates. (a) Mineralization fluorescence on all substrates (in green), scale bar = 200 μm (b) Adiponectin fluorescence on all substrates (in red), scale bar = 100 μm (c) Quantification of mineralization fluorescence (one-way ANOVA with post hoc Tukey HSD test, n ≥ 3 samples, at least 5 images per sample, ^#p < 0.05 relative to flat BMG) (d) Quantification of adiponectin fluorescence (one-way ANOVA with post hoc Tukey HSD test, n = 3 samples, at least 15 images per sample, ^#p < 0.05 relative to 200 nm BMG). DAPI was used for nuclear staining (in blue). Error bars represent standard error of the mean (SEM).