Figure 5

Mineralization with experimental modifications. (a) Mineralization fluorescence for aspect ratio modifications of 200 nm Pt-BMG (in green), DAPI was used for nuclear staining (in blue). scale bar = 100 μm (b) Quantification of mineralization fluorescence on modifications (one-way ANOVA with post hoc Tukey HSD test, n = 3 samples, ^#p < 0.05 in comparison to flat BMG). (c) Theoretical stiffness values. (d) YAP fluorescence of hMSCs cultured on flat Pt-BMG and 200 nm BMG pressed at 7KN (in green), scale bar = 100 μm (e) Calculation of YAP nuclear localization fluorescence (student’s t-test, n = 3 samples, at least 40 cells per sample *p < 0.05). (f) Quantification of hMSC circularity (student’s t-test, n > 40 cells, *p < 0.05). Error bars represent standard error of the mean (SEM).