Figure 1
Cd activates the Fas/FasL apoptotic signaling pathway in neuronal cells. Primary rat cerebral cortical neurons and PC12 cells incubated with Cd (0, 5, 10, or 20 µM) for 24 h (A), or with Cd (10 µM) for 0, 6, 12, or 24 h (B) were harvested, Fas, FasL, FADD, and cleaved caspase-8 protein levels were assessed by western blot analysis using the relevant antibodies. Blots for Fas, FasL, FADD and cleaved caspase-8 were semi-quantified using Image LabTM software. β-actin was used as internal control. Similar results were observed in at least three independent experiments. Data are expressed as mean ± SD (n = 3) relative to control. *P < 0.05 and **P < 0.01.
