Figure 2 | Scientific Reports

Figure 2

From: Adipocytes induce distinct gene expression profiles in mammary tumor cells and enhance inflammatory signaling in invasive breast cancer cells

Figure 2

MDA-MB-231 cells show increased expression of pro-inflammatory genes and enhanced IL-8 and IL-6 secretion upon co-culture with differentiated 3T3-L1 adipocytes. MDA-MB-231 breast cancer cells were co-cultured either with 3T3-L1 adipocytes (CoAd), 3T3-L1 fibroblasts (CoFi), Adipocyte-conditioned medium (ACM) or alone (control) for 5 days. (a) After 5 days of co-culture, total RNA was extracted. Relative mRNA expression of selected genes was determined by performing quantitative RT-PCR. (b) Following 5 days of co-culture, supernatants were collected and cytokine expression was determined using ELISA Kits specifically detecting human IL-8 and IL-6. Data are presented as the means ± SD of triplicates from one representative experiment. Significance is relative to control unless indicated otherwise (*p < 0.05; **p < 0.005; ***p < 0.0005).

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