Figure 5 | Scientific Reports

Figure 5

From: Adipocytes induce distinct gene expression profiles in mammary tumor cells and enhance inflammatory signaling in invasive breast cancer cells

Figure 5

Increased expression of NF-kB target genes and secretion of hIL-8 is dependent on active NF-KB signaling in MDA-MB-231 breast cancer cells. (a) MDA-MB-231 breast cancer cells were co-cultured either with 3T3-L1 adipocytes (CoAd), 3T3-L1 fibroblasts (CoFi) or alone (control) for 5 days in presence or absence of 20 µM JSH-23. mRNA expression levels of selected NF-κB-target genes were analysed by qRT-PCR analyses. (b) Following 5 days of co-culture with or without 20 µM of JSH-23 present, supernatants were collected and cytokine levels were determined using ELISA Kit specifically detecting human IL-8. Data are presented as the means ± SD of triplicates from one representative experiment. Significance is relative to basal conditions (JSH-23 vs DMSO) unless indicated otherwise (*p < 0.05; **p < 0.005; ***p < 0.0005).

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