Figure 4 | Scientific Reports

Figure 4

From: Dropping anchor: attachment of peptidylarginine deiminase via A-LPS to secreted outer membrane vesicles of Porphyromonas gingivalis

Figure 4

Detection of A-LPS-modified proteins in PPAD sorting type II isolates. P. gingivalis was cultured in BHI and, subsequently, the cells and growth medium fractions were separated by centrifugation. Proteins in both fractions were separated by LDS-PAGE and analyzed by Western blotting using an A-LPS-specific monoclonal antibody (green signal) and a PPAD-specific antibody (red signal) as indicated for Fig. 3. The name of the PPAD sorting type I isolate used as a control is underlined. Molecular weights of marker proteins are indicated. The full-length blot is presented in Figure S1.

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