Table 4 A Set of Primers Used for RT-PCR and Direct Sequencing in NS5A and NS5B region.
Name | Nucleoside Sequences (5′ → 3′) | Nt Position* | |
|---|---|---|---|
NS5A region | |||
NS5A_L | AGGGATGTTTGGGACTGG | 6273–6290 | 1st PCR, sense |
NS5A_R | CCGTCACGTAGTGGAAATC | 6633–6651 | RT, 1st PCR, anti-sense |
NS5B region | |||
HCV-16L | GTCCTGGAAGGACCCGGACTACGTC | 7238–7262 | 1st PCR, sense |
HCV-16.5 L | AGCTGGTGAGGACGTCGTCTGCTGC | 7574–7598 | 2nd PCR, sense |
HCV-17L | GCAGAAGAAGGTCACCTTTGACAGA | 7742–7766 | 2nd PCR, sense |
HCV-18L | GGTGAATACCTGGAAATCAAAGAAA | 8210–8234 | 2nd PCR, sense |
HCV-19L | CTCGCACGGGCTGCGTGGGAGACAG | 8772–8796 | 2nd PCR, sense |
HCV-16R | TCATCTCCTTGAGCACGTCCCGGTA | 7788–7812 | 2nd PCR anti-sense |
HCV-17R | TCGGGGGCCAAGTCACAACATTGGT | 8317–8341 | 2nd PCR, anti-sense |
HCV-18R | AGAAATGAGTCATCAGAATCATCCT | 8862–8886 | 2nd PCR, anti-sense |
HCV-18.5 R | TGGCCTGGAGTGGTTAGCTCCCCGT | 9375–9399 | 2nd PCR, anti-sense |
HCV-19R | AGGGAATGGCCTATTGGCCTGGAGT | 9389–9413 | RT, 1st PCR, anti-sense |
