Figure 3
Demonstration of the utility of STMs for detection of sample swaps and cross-contamination. (a) Map illustrating the results of the sample swapping experiment. Samples (columns) and STMs (rows) are ordered such that pie charts located on the off-diagonal indicate swapped samples. Double-headed arrows highlight identified sample mix-ups, which were in all cases in agreement with the single-blinded experimental design (Supplementary Table S3). (b) Graphics summarizing the results of the cross-contamination experiment. The upper left panel displays the ordination of the samples, showing the expected trend based on the composition of the admixtures. The lower plot shows pie charts of the distribution of reads assigned to the STMs for both samples. The upper right panel shows the relationship between estimated carry-over and theoretical admixture proportions. The solid line shows the 1:1 diagonal. Data from technical replicates are shown.
