Figure 4
Evaluation of the STMs added at the point of DNA and case demonstration of unanticipated cross-contamination. (a) Heatmap of normalized read counts for each of the spike-in controls in the different samples. Blue and red boxes indicate spike-in controls assigned to the majority and minority STMs for each sample, respectively. Note that spike-in controls may be marked with both blue and red boxes in cases where spike-in controls are shared between the majority and minority STMs. (b) Visualization of the inferred pattern of cross-contamination based on minority STMs. The width of the arrows is scaled according to the estimated percentage of between-sample carry-over. Arrows are colored based on the number of non-shared spike-in controls between the source and contaminated samples/wells.
